EDTA on Ultisil AQ-C18
Sample Solution
| Column | Ultisil AQ-C18, 3μm, 4.6×150mm (P/N 00207-21041) |
| Mobile Phase | Acetonitrile:Buffer(6ml 10% C16H37NO, add water to 200ml, adjust pH to 6.5 with phosphoric acid):Water=20:20:60 |
| Detection | 254nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |