Emtricitabine on Ultisil Plus C18
Mixed standard solution
Gradient Program
| Time（min） | A% | B% |
| 0 | 98 | 2 |
| 10 | 80 | 20 |
| 20 | 40 | 60 |
| 35 | 20 | 80 |
| 40 | 20 | 80 |
| 41 | 98 | 2 |
| 50 | 98 | 2 |
| Column | Welch Ultisil Plus C18 4.6×250mm，5μm (P/N: 00260-31043) |
| Mobile Phase | Mobile phase A: 1.54g/L ammonia acetate solution, adjust pH to 4.0 with glacial acetic acid Mobile phase B: acetonitrile |
| Detector | 280nm |
| Temperature | 40℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 20µl |