Ephedra Sinica on Ultisil Polar RP
Reference substance
| Column | Welch Ultisil Polar RP 4.6×150mm，5μm (P/N: 00215-31043) |
| Mobile Phase | Methanol-0.01mol/L potassium dihydrogen phosphate solution (pH adjusted to 2.5 with phosphoric acid) = 17:83 |
| Detector | 210 nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |