Esomeprazole Sodium Determination on Ultisil XB-C18
Gradient Program
| Time（min） | A% | B% |
| 0 | 100 | 0 |
| 10 | 80 | 20 |
| 30 | 0 | 100 |
| 31 | 100 | 0 |
| 45 | 100 | 0 |
| Column | Welch Ultisil XB-C18 4.6×250mm，5μm (P/N: 00201-31043) |
| Mobile Phase | A: Acetonitrile-phosphate buffer (pH 7.6) - water (10:10:80) B: Acetonitrile-phosphate buffer (pH 7.6) - water (80:1:19) |
| Detector | 302nm |
| Temperature | / |
| Flow Rate | 1ml/min |
| Injection Volume | 10ul |