Eudragit on Ultisil XB-NH2

Blank(mobile phase)

Column Ultisil XB-NH2, 5μm, 4.6×250mm (P/N 00204-31043)
Mobile Phase KH2PO4(1.702g dissolved into 500ml with water): Tetrahydrofuran=25:75
Detection 215nm
Temperature 30℃
Flow Rate 1.0ml/min
Injection Volume 20ul