Ultisil Cellu-DR-MBLSW Isomer Analysis Method Development Report
Test Report
Chromatography conditions:
Column |
Ultisil Cellu-DR（4.6×300mm,5μm） |
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Mobile Phase |
Phase A: 0.1% formic acid in water Phase B: 0.1% formic acid in acetonitrile |
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Injection Volume |
10 μL |
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Column temperature |
25 ℃ |
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Flow Rate |
0.8 ml/min |
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Detector |
UV |
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Detect wavelength |
254 nm |
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Elution procedure |
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- 1. Spectra and data
- 1) Blank solvent analysis spectrum:
- 2) 0361167 Analysis spectrum of control solution:
Serial number |
Peak name |
Retention time (min) |
Peak Area(mAU*min) |
S/N |
Relative peak area(%) |
Resolution(EP) |
Number of plates(EP) |
Asymmetry(EP) |
1 |
0361167 |
32.148 |
234.418 |
380.0 |
100.00 |
N.a. |
8243 |
1.28 |
Sum |
|
234.418 |
|
100.00 |
0.00 |
|
|
- 3) 0355439 Control solution analysis spectrum:
Serial number |
Peak name |
Retention time (min) |
Peak Area(mAU*min) |
S/N |
Relative peak area(%) |
Resolution(EP) |
Number of plates(EP) |
Asymmetry(EP) |
1 |
0355439 |
45.703 |
280.178 |
40.1 |
100.00 |
N.a. |
4906 |
0.77 |
Sum |
|
28.178 |
|
100.00 |
0.00 |
|
|
- 4) 0355424 Control solution analysis spectrum:
Serial number |
Peak name |
Retention time (min) |
Peak Area(mAU*min) |
S/N |
Relative peak area(%) |
Resolution(EP) |
Number of plates(EP) |
Asymmetry(EP) |
1 |
0355424 |
36.302 |
271.456 |
146.3 |
100.00 |
N.a. |
7990 |
1.45 |
Sum |
|
271.456 |
|
100.00 |
0.00 |
|
|
- 5) 0355437 Analysis spectrum of control solution:
Serial number |
Peak name |
Retention time (min) |
Peak Area(mAU*min) |
S/N |
Relative peak area(%) |
Resolution |
Number of plates(EP) |
Asymmetry |
1 |
36.558 |
25.210 |
62.7 |
8.66 |
1.96 |
8730 |
N.a. |
|
2 |
0355437 |
40.378 |
265.876 |
411.7 |
91.34 |
N.a. |
4791 |
0.81 |
Sum |
|
291.066 |
|
100.00 |
1.96 |
|
|
- 6) Mixed control solution analysis spectrum:
Serial number |
Peak name |
Retention time (min) |
Peak Area(mAU*min) |
S/N |
Relative peak area(%) |
Resolution |
Number of plates(EP) |
Asymmetry |
1 |
0361167 |
32.392 |
53.865 |
135.0 |
19.62 |
2.91 |
8720 |
1.18 |
2 |
0355424 |
36.682 |
71.541 |
155.7 |
26.06 |
2.08 |
8793 |
1.32 |
3 |
0355437 |
40.775 |
77.732 |
39.4 |
28.31 |
2.08 |
4669 |
N.a. |
4 |
0355439 |
46.067 |
71.407 |
30.0 |
26.01 |
N.a. |
4621 |
N.a. |
Sum |
|
274.545 |
|
100.00 |
7.07 |
|
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Conclusion:
Welch column Ultisil Cellu-DR (4.6×300mm, 5μm) was used to analyze the four substances under this chromatographic condition. The separation between them was greater than 2, which met the analysis requirements.