Ultisil MM C18-SCX-Analysis Method Development Report for Tranexamic Acid & Aminomethylbenzoic Acid & p-Toluic Acid and p-Methylcyclohexanecarboxylic Acid
Test Report
Chromatography conditions:
Column |
Welch MM C18/SCX（4.6×250mm,5μm） |
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Mobile Phase |
Phase A: triethylamine phosphate buffer (pH=3.0) Phase B: acetonitrile |
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Column Temperature |
30℃ |
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Detector |
UV |
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Injection Volume |
10 μL |
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Flow Rate |
1.0 ml/min |
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Detect wavelength |
220 nm |
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Elution procedure |
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Note |
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Chromatogram and Data:
1) Blank solvent analysis spectrum
2) Mixed reference solution full range analysis spectrum:
- 3) Partial range analysis spectrum of mixed control solution
Conclusion:
The mixed control solution was analyzed using a Welch column Ultisil MM C18/SCX (4.6×250mm, 5μm) under this chromatographic condition. Tranexamic acid, aminobenzoic acid, p-toluic acid and p-methylcyclohexanecarboxylic acid were eluted in sequence with a separation degree of 2.97, 22.7 and 8.1, respectively, which all met the analysis requirements.