Ultisil PAH&Topsil C18-Unknown Compound Analysis Method Development Report
Test Report
Chromatography conditions:
Column 1 |
Welch Ultisil PAH（4.6×250mm,5μm） |
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Column 2 |
Topsil C18 （4.6×250mm,5μm ） |
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Mobile Phase |
0.005mol/L diammonium phosphate buffer-methanol = 25:75 |
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Column Temperature |
35℃ |
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Detector |
UV |
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Injection Volume |
20μL |
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Flow Rate |
1.0 ml/min |
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Detect wavelength |
280 nm |
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Elution procedure |
|
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Note |
/ |
Chromatogram and Data:
1) Ultisil PAH (4.6×250mm, 5μm) analysis results spectrum
Serial number |
Peak name |
Retention time (min) |
Peak Area(mAU*min) |
S/N |
Relative peak area(%) |
Resolution |
Number of plates(EP) |
Asymmetry |
1 |
Main peak 1 |
6.160 |
0.356 |
1.4 |
37.10 |
3.13 |
5209 |
1.40 |
2 |
Main peak 2 |
7.352 |
0.336 |
52.4 |
35.06 |
2.32 |
4837 |
1.31 |
3 |
Main peak 3 |
8.420 |
0.267 |
0.8 |
27.84 |
N.a. |
4519 |
1.35 |
Sum |
|
0.959 |
|
100.00 |
5.45 |
|
|
2) Topsil C18 (4.6×250mm, 5μm) analysis results spectrum
Serial number |
Peak name |
Retention time (min) |
Peak Area(mAU*min) |
S/N |
Relative peak area(%) |
Resolution |
Number of plates(EP) |
Asymmetry |
1 |
Main peak 1 |
6.330 |
0.378 |
19.3 |
38.67 |
4.31 |
10590 |
1.10 |
2 |
Main peak 2 |
7.528 |
0.323 |
27.9 |
33.03 |
1.51 |
9140 |
0.96 |
3 |
Main peak 3 |
7.990 |
0.277 |
24.3 |
28.30 |
N.a. |
11016 |
1.01 |
Sum |
|
0.978 |
|
100.00 |
5.81 |
|
|
Conclusion:
Welch column was used to analyze the system suitability solution under this chromatographic condition. The Ultisil PAH column (4.6×250mm, 5μm) showed better separation between the targets in the analysis results, while the Topsil C18 (4.6×250mm, 5μm) column met the requirements and had a high cost-performance ratio, meeting customer requirements.