Ultisil SiO2-4-Piperidinic Acid Analysis Method Development Report

Test Report

Chromatography conditions:

Column

Welch SiO2（4.6*250mm*5μm）

Mobile Phase

Phase A: 0.01mol/L potassium dihydrogen phosphate solution Phase B: acetonitrile

Column Temperature

35℃

Detector

Injection Volume

10μL

Flow Rate

1.0 ml/min

Detect wavelength

210 nm

Elution procedure

Time (min)	A phase%	B phase%
0	20	80
4	20	80
4.1	60	40
15	60	40
15.1	20	80
20	20	80

Note

Chromatogram and Data:

1. Blank solvent analysis spectrum

2. Mixed control solution analysis spectrum

3. Mixed control solution analysis spectrum

Conclusion:

The mixed control solution was analyzed under this chromatographic condition using a Welch column Ultisil SiO2 (4.6*250mm*5μm). The retention times of 4-pyridinecarboxylic acid and 4-piperidinic acid were 6.35min and 9.32min, respectively, and the separation degree between them was 19.2, both meeting the analysis requirements.