Test Report
Chromatography conditions:
Column |
Welch SiO2(4.6*250mm*5μm) |
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Mobile Phase |
Phase A: 0.01mol/L potassium dihydrogen phosphate solution Phase B: acetonitrile |
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Column Temperature |
35℃ |
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Detector |
UV |
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Injection Volume |
10μL |
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Flow Rate |
1.0 ml/min |
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Detect wavelength |
210 nm |
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Elution procedure |
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Note |
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Chromatogram and Data:
- 1. Blank solvent analysis spectrum
- 2. Mixed control solution analysis spectrum
- 3. Mixed control solution analysis spectrum
Conclusion:
The mixed control solution was analyzed under this chromatographic condition using a Welch column Ultisil SiO2 (4.6*250mm*5μm). The retention times of 4-pyridinecarboxylic acid and 4-piperidinic acid were 6.35min and 9.32min, respectively, and the separation degree between them was 19.2, both meeting the analysis requirements.