Ultisil XB-SCX-Urea & Bifonazole Analysis Method Development Report
Test Report
Chromatography conditions:
Column |
Welch Ultisil XB-SCX（4.6×250mm,5μm） |
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Mobile Phase |
Phase A : 6mmol/L ammonium acetate 0.03% TFA aqueous solution Phase B: chromatographic grade acetonitrile |
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Column Temperature |
25℃ |
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Detector |
UV |
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Injection Volume |
10μL |
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Flow Rate 1 |
1.0 ml/min |
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Flow Rate 2 |
1.3 ml/min |
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Detect wavelength |
195 nm |
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Elution procedure |
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Note |
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Chromatogram and Data:
1) Blank solvent analysis spectrum
2) Urea reference solution analysis spectrum:
- 3) Analysis spectrum of bifonazole control solution:
- 4) Mixed control solution analysis spectrum:
- 5) Analysis spectrum of mixed control solution at a flow rate of 1.3ml/min:
- 6) Sensitivity solution analysis spectrum at a flow rate of 1.3ml/min
Conclusion:
Using the Welch Ultisil XB-SCX column (4.6×250mm, 5μm) under these chromatographic conditions to analyze urea and bifonazole, the resolution between them is greater than 1.5. At a flow rate of 1.3 ml/min, the retention times for urea and bifonazole are 4.95 minutes and 11.1 minutes, respectively, meeting the analytical requirements.