Determination of Thiodicarb related substances by Ultisil XB-CN and Topsil CN
Test Report
Chromatography conditions:
Column |
Welch Ultisil®XB-CN（4.6×250mm，5μm） Welch Topsil® CN（4.6×250mm，5μm） |
Mobile Phase |
n-heptane/isopropyl alcohol/methylene chloride/acetonitrile=80/10/6/4 |
Column Temperature |
40℃ |
Detector |
UV |
Injection Volume |
5μL |
Flow Rate |
1.0 ml/min |
Detect wavelength |
240 nm |
Note |
/ |
Chromatogram and Data:
1) Welch Ultisil® XB-CN 202360607 test solution detection spectrum
Serial number |
Peak name |
Retention time (min) |
Peak Area(mAU*min) |
Peak height(mAu) |
Resolution(EP) |
Tailing factor |
Number of plates(EP) |
1 |
N.a. |
11.072 |
2749.687 |
174.883 |
0.0 |
1.159 |
12823 |
2) Welch Ultisil® XB-CN 202303118 test solution detection spectrum
Serial number |
Peak name |
Retention time (min) |
Peak Area(mAU*min) |
Peak height(mAu) |
Resolution(EP) |
Tailing factor |
Number of plates(EP) |
1 |
N.a. |
8.958 |
9.458 |
0.773 |
5.8 |
1.594 |
11605 |
2. |
N.a |
11.098 |
5322.349 |
321.348 |
0.0 |
1.165 |
11617 |
3 |
Sum |
|
|
|
|
|
|
- 3) Welch Ultisil® XB-CN spiked test solution detection chart
Serial number |
Peak name |
Retention time (min) |
Peak Area(mAU*min) |
Peak height(mAu) |
Resolution(EP) |
Tailing factor |
Number of plates(EP) |
1 |
N.a. |
8.978 |
39.772 |
3.278 |
6.0 |
1.041 |
12764 |
2. |
N.a |
11.070 |
3024.908 |
196.887 |
0.0 |
1.143 |
13247 |
3 |
Sum |
|
|
|
|
|
|
- 4) Topsil® CN 202360607 Test sample solution detection chart
Serial number |
Peak name |
Retention time (min) |
Peak Area(mAU*min) |
Peak height(mAu) |
Resolution(EP) |
Tailing factor |
Number of plates(EP) |
1 |
N.a. |
10.397 |
3028.524 |
200.291 |
0.0 |
1.232 |
12193 |
2 |
Sum |
|
|
|
|
|
|
- 5) Topsil® CN 202303118 spiked test solution detection chart
Serial number |
Peak name |
Retention time (min) |
Peak Area(mAU*min) |
Peak height(mAu) |
Resolution(EP) |
Tailing factor |
Number of plates(EP) |
1 |
N.a. |
8.040 |
10.595 |
1.118 |
7.2 |
1.114 |
16762 |
2 |
N.a. |
10.338 |
5283.361 |
336.957 |
0.0 |
1.238 |
11259 |
|
Sum |
|
|
|
|
|
|
- 6) Topsil® CN spiked test solution detection chart
Serial number |
Peak name |
Retention time (min) |
Peak Area(mAU*min) |
Peak height(mAu) |
Resolution(EP) |
Tailing factor |
Number of plates(EP) |
1 |
N.a. |
8.065 |
42.304 |
3.880 |
7.0 |
1.235 |
13307 |
2 |
N.a. |
10.353 |
2984.087 |
196.951 |
0.0 |
1.236 |
12014 |
|
Sum |
|
|
|
|
|
|
Conclusion:
Under these chromatographic conditions, Ultisil ® XB-CN（4.6×250mm，5μm）and Topsil® CN（4.6×250mm，5μm）, can meet the customers’ requirements.