Determination of Acetaminophen in Pork

Range of Application

It is suitable for the determination of acetaminophen residue in muscle, liver and kidney tissue of cattle, sheep and pig (pork was selected as matrix in this experiment).

Purification Steps

SPE column: Welchrom®BRP column, specification: 60 mg/3mL.

Activation: 3 mL methanol, 3 mL water, discard

Sample loading: load all the samples, discard

Flushing: 3 mL water, 3 mL 5% methanol, discard

Elution: 4 mL methanol, blow the eluent to dry at 40℃ nitrogen, and add it to 1 mL with the mobile phase

Chromatographic Conditions

Column: UItisil®XB-C18,4.6×250 mm, 5μm.

Mobile phase: methanol: 0.05 mol/L ammonium acetate solution=2:8

Flow rate: 1 mL/min

Column temperature: 30℃

Injection volume: 50 μL

Wavelength: 250 nm

Chromatogram or Spike Recovery Rate

Chromatogram of 0.1mg/L Acetaminophen

Name	Rt	Area	Height	Relative peak area	Tailing factor	Plate
_	min	mAU*min	mAU	%	EP	(USP)
Acetaminophen	7.313	0.303	2.140	100	1.01	16844

Figure 2. Blank Chromatogram of Pork Sample

Figure 3. Chromatogram of Pork Sample at 0.02mg/kg

Table 1 Spike Recovery Rate

Name	Rt mg/kg	Average Recovery Rate %	RSD% （n=2）
Acetaminophen	0.02	97	4.70

Ordering Information

P/N	Product	Specification
00522-20009	SPE column	Welchrom® BRP, 60mg/3mL, 50pk
00837-05006	50mL centrifuge tube with screw mouth and apical bottom	Disposable centrifuge tube with flat cap, and conical bottom, RCF12000xg, 15 mL, 50/ pk without sterilization
00837-05002	15mL centrifuge tube with screw mouth and apical bottom	Disposable centrifuge tube with flat cap, and conical bottom, RCF12000xg, 15 mL, 50/pk without sterilization
00824-31001	Welch SPE manifold	12 ports
00821-32291	Caps and septa	Pre-slit red PTFE/white Silicone septa, 9mm blue short screw-thread polypropylene cap, 6mm centre hole, 100/pk
00821-40927	Vials	2ml wide opening short screw-thread vial with write-on spot, clear, 11.6*32mm, 100/pk
00201-31043	HPLC Column	Ultisil® XB-C18, 4.6×250mm, 5μm