Determination of the Vitamin B6 in Food
Vitamin B6, which includes pyridoxol, pyridoxal and pyridoxamine, is water-soluble in the form of phosphate in human body. As a component of some coenzymes in human body, it participates in many metabolic reactions, especially in amino acid metabolism. Clinical applicable vitamin B6 can prevent pregnancy vomiting and radiation vomiting. Yeast, liver, grain, meat, fish, eggs, beans and peanuts are particularly rich in vitamin B6.
Chromatographic Condition
| Column | Ultisil® AQ-C18 | （4.6×150mm，5μm) | |
| Mobile Phase | Time（min） | Mobile phase A（%） | Methanol（%） |
| 0 | 100 | 0 | |
| 20 | 100 | 0 | |
| 30 | 65 | 35 | |
| 45 | 62 | 38 | |
| 46 | 50 | 50 | |
| 50 | 50 | 50 | |
| 51 | 100 | 0 | |
| 75 | 100 | 0 | |
| Wavelength | Excitation:293nm | Emission: 395nm | |
| Column Temp. | 30℃ | ||
| Flow Rate | 1.0ml/min | ||
| Injection Volume | 10μL |
Chromatogram and Data
| Substance | Retention time | Area | Height | Plates | Tailing factor | Resolution |
| Pyridoxal | 15.295 | 55641163 | 2708855 | 12581 | 0.999 | - |
| Pyridoxol | 22.436 | 87149062 | 2970285 | 13341 | 0.996 | 10.801 |
| Pyridoxamine | 36.602 | 73230997 | 9202969 | 445781 | 1.087 | 28.438 |
| Total | _ | 216021223 | 14882108 | _ | _ | _ |
Conclusion
Using AQ-C18(4.6×150 mm,5μm) under this chromatographic condition can meet the test requirements.