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Welch Materials, prioritizing product stability, has developed a new series of products, Welchrom® 96-Well Plates, through over a year of technical research and process testing. This series of products is primarily designed for customers involved in the pre-treatment of biological samples, aiming to provide customers with comprehensive solutions and customized services, making the pre-treatment of biological samples simpler and more efficient.
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Welchrom® 96-Well Plates are divided into two major series:
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Welchrom® 96-Well Plates – Solid Phase Extraction (SPE) Plates
Adopting an integrated design, these plates are suitable for processing large-volume biological samples. They enable concentration after elution of large-volume samples, enhancing detection sensitivity. Each well is filled with 10mg to 60mg of sorbent material.
2. Welchrom® 96 Microporous Extraction Plate
Adopting an integrated design, this plate is suitable for extracting target compounds from small-volume biological samples. It requires only a small amount of elution solvent to elute the target substances, eliminating the need for concentration and reconstitution steps. Each well is filled with 2mg to 5mg of sorbent material.
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Product Features:
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Low Sorbent Volume, High Throughput, Less Prone to Clogging:
- Compared to traditional SPE columns, this product has the advantages of low sorbent volume, high throughput, and reduced susceptibility to clogging.
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Diverse Adsorbent Types, Varied Product Specifications:
- The product offers a diversity of adsorbent types and specifications to meet various experimental needs.
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Excellent Reproducibility, Stability, and Reliability:
- Demonstrates good reproducibility and stability, providing reliable results and reducing matrix effects in LC-MS/MS analysis.
Application Case:
Product: Welchrom® BRP 96-Well Plates, 5mg/1mL.
Project: Illustration using the example of determining the levels of Hydrocortisone, Testosterone, and Progesterone in serum.
1. Sample Preparation:
- Sample:100μL serum sample + 100μL methanol-water (50/50) containing 1% formic acid.
- Sample with Standard Addition:100μL serum sample + 100μL methanol-water (50/50) containing 1% formic acid + 10μL 1μg/mL mixed standard.
- Blank:100μL water + 100μL methanol-water (50/50) containing 1% formic acid.
- Blank with Standard Addition:100μL water + 100μL methanol-water (50/50) containing 1% formic acid + 10μL 1μg/mL mixed standard.
2. Column Pass-Through Steps:
- 96 Microporous Extraction Plate: Welchrom® BRP 96-Well Plates, 5mg/1mL.
- Activation:200μL methanol, 200μL water;
- Sample Loading: Preprocess samples;
- Washing: Wash with 400μL methanol-water solution (35/65);
- Elution: With 200μL of 1% formic acid in methanol, collecting in a 96-well plate, followed by UHPLC-MS/MS detection.
3. Chromatographic Conditions
UPLC Conditions:
- Column: Ultisil® XB-C18 (00201-21010), 3μm, 2.1×50mm.
- Guard Column Core: Ultimate® XB-C18 (00808-23001), Core: 3µm, 120Å, 2.1×10mm.
- Mobile Phase: A: 0.1% formic acid aqueous solution, B: 0.1% formic acid in acetonitrile (40/60).
- Column Temperature: 30℃.
- Flow Rate: 0.2mL/min.
- Injection Volume: 2µL.
Mass Spectrometry Conditions:
- Ion Source: Electrospray Ionization (ESI).
- Scan Mode: Positive ion scan.
- Detection Mode: Multiple Reaction Monitoring (MRM) mode.
- Ion Spray Voltage: 5500V.
- Ion Source Temperature: 500℃.
Gas Curtain Gas (CUR): 10 psi. Drying Gas (GS1): 55 psi. Auxiliary Gas (GS2): 50 psi.
For other mass spectrometry parameters, refer to Table 2.
Table 1: Multiple Reaction Monitoring (MRM) Parameters
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Chromatogram and actual sample test results
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Table 2: Spike Recovery Rates
Compound | Spiked Concentration (ng/mL) | Average Recovery Rate (%) | RSD (N≥50%) |
Hydrocortisone | 50ng/mL | 100.80% | 7.49% |
Testosterone | 50ng/mL | 104.38% | 9.63% |
Progesterone | 50ng/mL | 90.63% | 9.00% |
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Product information
Welchrom® 96-Well Solid Phase Extraction Plate
P/N | Specification |
01140-01608 | Welchrom® BRP 96-well plates, 60mg/2mL,1pk |
01140-01608 | Welchrom® BRP 96-well plates, 50mg/2mL,1pk |
01140-01308 | Welchrom® BRP 96-well plates, 50mg/2mL,1pk |
01140-01108 | Welchrom® BRP 96-well plates, 10mg/2mL,1pk |
01140-02608 | Welchrom® P-SAX 96-well plates, 30mg/2mL,1pk |
01140-02508 | Welchrom® P-SAX 96-well plates, 10mg/2mL,1pk |
01140-03308 | Welchrom® P-SCX 96-well plates, 30mg/2mL,1pk |
01140-03108 | Welchrom® P-SCX 96-well plates, 10mg/2mL,1pk |
01140-04108 | Welchrom® P-WCX 96-well plates, 10mg/2mL,1pk |
01140-04208 | Welchrom® P-WCX 96-well plates, 20mg/2mL,1pk |
01140-05308 | Welchrom® P-WAX 96-well plates, 30mg/2mL,1pk |
01140-05108 | Welchrom® P-WAX 96-well plates, 10mg/2mL,1pk |
01140-06508 | Welchrom® C18E 96-well plates, 50mg/2mL,1pk |
01140-06308 | Welchrom® C18E 96-well plates, 30mg/2mL,1pk |
01140-06258 | Welchrom® C18E 96-well plates, 25mg/2mL,1pk |
01140-06108 | Welchrom® C18E 96-well plates, 10mg/2mL,1pk |
01140-07608 | Welchrom® PLR (phospholipid removal) 96-well plates, 60mg/2mL, 1pk |
01140-07508 | Welchrom® PLR (phospholipid removal) 96-well plates, 50mg/2mL, 1pk |
01140-07308 | Welchrom® PLR (phospholipid removal) 96-well plates, 30mg/2mL, 1pk |
Welchrom® 96 Microwell Extraction Plate
P/N | Specification |
01140-01059 | Welchrom® BRP 96-well plates, 2mg/1mL,1pk |
01140-01029 | Welchrom® BRP 96-well plates, 2mg/1mL,1pk |
01140-02059 | Welchrom® P-SAX 96-well plates, 5mg/1mL,1pk |
01140-02029 | Welchrom® P-SAX 96-well plates, 2mg/1mL,1pk2mg/1mL,1pk |
01140-03059 | Welchrom® P-SCX 96-well plates, 5mg/1mL,1pk |
01140-03029 | Welchrom® P-SCX 96-well plates, 2mg/1mL,1pk |
01140-04059 | Welchrom® P-WCX 96-well plates, 5mg/1mL,1pk |
01140-04029 | Welchrom® P-WCX 96-well plates, 2mg/1mL,1pk |
01140-05059 | Welchrom® P-WAX 96-well plates, 5mg/1mL,1pk |
01140-05029 | Welchrom® P-WAX 96-well plates, 2mg/1mL,1pk |
01140-06059 | Welchrom® C18E 96-well plates, 5mg/1mL,1pk |
01140-06029 | Welchrom® C18E 96-well plates, 2mg/1mL,1pk |
01140-07059 | Welchrom® PLR (phospholipid removal) 96-well plates, 5mg/1mL, 1pk |
01140-07029 | Welchrom® PLR (phospholipid removal) 96-well plates, 2mg/1mL, 1pk |
Note: For other specifications, customization is available upon contact.
Storage Conditions and Shelf Life:Store at room temperature; under these conditions, the shelf life is 3 years.