Determination of Hormones in Essence Water - Group A Experiment Report
Test Report
Chromatography conditions:
Column |
Welch Xtimate® UHPLC C18 2.1×150mm，1.8μm |
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Mobile Phase |
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Column Temperature |
40℃ |
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Detector |
UV |
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Injection Volume |
5μL |
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Flow Rate |
0.3 ml/min |
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Note |
This test uses the matrix curve. |
Chromatogram and Data:
1) Blank
- 2) Sample solution
- 3) 10 ng/mL matrix curve
- 4) Sample spiked, 10 ng/mL.
Conclusion:
Using Welch Xtimate® UHPLC C18 2.1×150mm, 1.8μm, the recovery rates of dexamethasone, betamethasone, prednisone, difluprednate, hydrocortisone, cortisone, methylprednisolone, and flumethasone in cosmetic water are 106.1%, 97.5%, 93.2%, 89.4%, 87.4%, 108.5%, 98.5%, and 94.9% respectively. The sample recovery rates meet the requirements.