Separation method development and reporting using Xtimate C18-DA
Test Report
Chromatography conditions:
Column |
Xtimate C18（4.6×250mm,5μm） |
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Mobile Phase |
0.1% Formic Acid in Water: Acetonitrile |
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Injection Volume |
5 μL |
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Flow Rate |
1ml/min |
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Detector |
DAD |
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Detect wavelength |
254nm |
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Elution procedure |
|
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Note |
N/A |
- 1. Spectra and data
- (1) Full-range detection spectrum of sample solution
Signal: DAD1 A, Sig=254,4 Ref=360,100
Serial number |
Retention time (min) |
Peak Area |
Area percentage |
Resolution(USP) |
Tailing factor |
Number of plates(usp) |
1 |
18.39 |
1014.28 |
30.80 |
|
0.81 |
200216.0 |
2 |
18.68 |
326.79 |
9.923 |
1.10 |
0.91 |
193280.3 |
3 |
20.04 |
229.68 |
6.974 |
7.83 |
1.07 |
205979.7 |
4 |
20.26 |
177.53 |
5.391 |
1.31 |
0.96 |
220968.7 |
5 |
21.93 |
378.27 |
11.49 |
9.02 |
0.76 |
198527.2 |
6 |
22.07 |
117.03 |
3.554 |
0.53 |
1.84 |
79144.9 |
7 |
22.47 |
1049.59 |
31.87 |
1.62 |
1.03 |
234886.7 |
(2) Local amplification detection spectrum of sample solution
Signal: DAD1 A. Sig=254.4 Ref=360,100
Serial number |
Retention time (min) |
Peak Area |
Area percentage |
Resolution(USP) |
Tailing factor |
Number of plates(usp) |
1 |
18.39 |
1014.28 |
30.80 |
|
0.81 |
200216.0 |
2 |
18.68 |
326.79 |
9.923 |
1.70 |
0.91 |
193280.3 |
3 |
20.04 |
229.68 |
6.974 |
7.83 |
1.07 |
205979.7 |
4 |
20.26 |
177.53 |
5.391 |
1.31 |
0.96 |
220968.7 |
5 |
21.93 |
378.27 |
11.49 |
9.02 |
0.76 |
198527.2 |
6 |
22.07 |
117.03 |
3.554 |
0.53 |
1.84 |
79144.9 |
7 |
22.47 |
1049.59 |
31.87 |
1.62 |
1.03 |
234886.7 |
Conclusion:
Using Welch Xtimate® C18 (4.6×250mm, 5μm) under this chromatographic condition, the four target peaks were separated from each other, meeting the customer's detection requirements