Separation of SK-SM1 and its isomers using Ultisil Amy-D
Test Report
Chromatography conditions:
|
Column |
Ultisil® Amy-D（4.6×250mm，5μm） |
|
Mobile Phase |
n-hexane/isopropyl alcohol/trifluoroacetic acid=80/20/0.1 |
|
Detector |
UV |
|
Injection Volume |
20 μL |
|
Flow Rate |
1.0 ml /min |
|
Detection wavelength: |
254 nm |
|
Column Temperature |
30℃ |
|
Note |
/ |
Chromatogram and Data:
- Detection amplification chart of mixed standard solution
|
Peak Name |
Retention time(min) |
Area(mAU*s) |
Height(mAU) |
Resolution(EP) |
Tailing Factor(EP) |
Number of theoretical plates(EP) |
SNR |
|
SK-SM1 |
11.293 |
51106.878 |
1469.095 |
5.8 |
1.218 |
2291 |
12083.72 |
|
SK-SM1-Z |
17.160 |
168.469 |
4.116 |
0.0 |
0.997 |
3906 |
33.859 |
|
Sum |
|
|
|
|
|
|
|
2. Mixed standard solution detection full-scale spectrum
Conclusion:
Under these chromatographic conditions, Welch Ultisil® Amy-D（4.6×250mm，5μm）can meet the detection requirements.