Chiral Separation of Remdesivir Isomers

2019-nCoV corona virus pandemic is exploding all over the world, how to cure it has become the most important and urgent matter at present. Remdesivir, developed by Gilead, which is considered as one of the new drugs to against novel coronaviruses, now is in clinical trial stage.

Applications of Remdesivir chiral isomer separation.

Sample

Remdesivir chiral isomer

Chromatographic condition

Column：Welch Ultisil® Cellu-DR（4.6 x 250 mm, 5 μm）
Detection wavelength：254 nm

Column temperature：30 ºC

Flow rate：0.6 mL/min
Injection volume：10 μL

Sample solution preparation:

Prepared with 50% acetonitrile to get a solution containing 0.18 mg sample per 1 mL.

Chromatogram and data:

Sample Solution (full scale)

Sample Solution (part of the scale)

RT	Area	Plates	Tailing	Resolution
13.393	1046378	11787	1.011	3.869
14.683	1128288	10665	1.043	2.431

Conclusion

Using Ultisil® Cellu-DR（4.6×250mm，5μm）under this chromatographic condition can meet the detection requirements