News
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Can Normal-Phase Chromatography Be Combined with Mass Spectrometry?
Recently, I have been involved in a project focused on the development of isomers of lycopene, a natural pigment found in food that belongs to the carotenoid family. Over the... -
LP-C18 : Can it Separate Isomers and Chiral Diastereomers?
Following the previous article “1Can LP Columns Only Resist Strong Acids?” this article takes a user-centric approach, guiding everyone in a clear and in-depth exploration to understand the manufacturer’s design... -
Chromatographic Column Selection in HPLC Method Development (Part 1)
In the development of reversed-phase liquid chromatography (RPLC) methods, the selection of chromatographic columns is generally approached through two methods: one based on practical experience and the other rooted in... -
Let you know our Ultisil ODS-3 Column
Ulisil ODS-3 column is packed with high water-resistance octadecyl reversed-phase packing material. This packingmaterial is strictly endcapped with hydrophilic groups, which brings perfect peaks and low adsorption for both alkalineand... -
Determination of Cis-trans Isomers in Vitamin K1
Vitamin K1, also known as the plant methylnaphthoquinone, chlorphenoquinone, chlorphenoquinone, 2-methyl-3- phytol-1,4-naphthoquinone, is a polycyclic aromatic ketone and fat-soluble vitamin. Extensively existing in natural green plants, Vitamin K1 is stable... -
How to Select Guard Columns and Pre-columns?
Inline Filters As in-line filters, pre-columns and guard columns have many similarities and differences simultaneously. ONE How to Differentiate Pre-columns From Guard Columns? Being connected before column, both pre-columns and... -
Q&A for Ion-Pairing Reagent
Ion-Pairing Reagent Ion-pairing reagent, a kind of reagent used in lab, can strengthen the retention of ion samples if added in mobile phase of reverse-phase chromatography. Ion-pairing reagents can improve... -
SEC (Size Exclusion Chromatography)
Overview SEC (Size Exclusion Chromatography) is a technique that separates molecules of interest by their molecular size. There are 2 types of SEC separation known as Gel Filtration Chromatography (uses... -
Reasons for High Efficiency of Core-shell Column
Core-shell column shows high column efficiency, low back pressure, high sensitivity, but majority of people may not know the reasons. In order to understand the characteristics of the core and... -
Review of Webinar: HPLC Method Development and Validation
About Webinar Last friday, Webinar about HPLC method development and validation was held online. Webinar Content a. Tradition strategy for HPLC method development and a survey of automated tools and... -
If pH<2, pay attention to the selection of column
Many analysts will adjust the peak shape and retention time by changing pH during the method development. Because the change of pH will exert various influences on chromatographic behavior of... -
How to Select a Suitable Column?
When you look up the pharmacopoeia method, you may find that it only stipulates the bonding phase of column and the information of other columns is not available. Therefore, you...
