Determination of Cis-trans Isomers in Vitamin K1
Vitamin K1, also known as the plant methylnaphthoquinone, chlorphenoquinone, chlorphenoquinone, 2-methyl-3- phytol-1,4-naphthoquinone, is a polycyclic aromatic ketone and fat-soluble vitamin. Extensively existing in natural green plants, Vitamin K1 is stable in the air and moisture, but it will be decomposed in the sun.
As the necessary substance for liver synthesis factors II, VII, IX, X, Vitamin K1 is mainly used for the treatment of hemorrhagic diseases caused by vitamin K deficiency.
Constitutional Formula
Chromatographic Condition
Column: Topsil®Silica（4.6×300mm，5μm)
Mobile phase: Petroleum ether (60~90℃)/n-pentanol =2000/2.5;
Wavelength: 254nm
Column Temp：40℃
Flow rate：0.6ml/min
Injection volume：10μL
Reference Substance Solution
| Peak | Rt | Area | Height | Plates | Resolution | Tailing factor |
| 1 | 13.274 | 3353330 | 276291 | 26482 | 0.00 | 1.011 |
| 2 | 14.613 | 22465941 | 1328178 | 17015 | 3.46 | 1.417 |
| Total | _ | 25819271 | 1604469 | _ | _ | _ |
Test Sample Solution
| Peak | Rt | Area | Height | Plates | Resolution | Tailing factor |
| 1 | 13.274 | 6692432 | 536722 | 25421 | 0.00 | 1.049 |
| 2 | 14.619 | 21679771 | 1295948 | 17458 | 3.54 | 1.387 |
| Total | _ | 28372203 | 1832670 | _ | _ | _ |
Conclusion
Using Topsil® Silica（4.6×300mm，5 μm) under this chromatographic condition can meet the test requirements.