Introduce the principle of amino columns (nh2 columns) and the guidelines for optimizing amino column performance in reversed-phase and normal-phase mode.
In this article, we explore how particle size affects resolution in liquid chromatography through two real-world applications, and analyze why these cases show different results from common belief.
This article introduces dead volume in HPLC: its definition, calculation methods, and tips to reduce it for better peak shapes and chromatographic efficiency.