Knowledge Base
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Issues and Solutions to the Use of Ion-Pairing Reagents
Explore the mechanism of ion-pairing reagents, and common issues and solutions when using ion-pairing chromatography. -
Amino (NH2) column: How to keep high performance?
Introduce the principle of amino columns (nh2 columns) and the guidelines for optimizing amino column performance in reversed-phase and normal-phase mode. -
Does Smaller Column Particle Size Always Lead to Better Resolution in Liquid Chromatography?
In this article, we explore how particle size affects resolution in liquid chromatography through two real-world applications, and analyze why these cases show different results from common belief. -
What is the Dead Volume of A Column?
This article introduces dead volume in HPLC: its definition, calculation methods, and tips to reduce it for better peak shapes and chromatographic efficiency. -
Optimizing Phosphate Buffer Solution: Preparation Methods and Case Studies
This article covers the role of phosphate buffer solutions in maintaining pH stability for HPLC, detailing preparation methods that enhance consistency in peak separation results.
