Knowledge Base
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Gradient Optimization in Liquid Chromatography
In this article, we explore key considerations in gradient method development, and witness how optimizing gradient elution in HPLC is key to achieving efficient separation through a real-world case study. -
Issues and Solutions to the Use of Ion-Pairing Reagents
Explore the mechanism of ion-pairing reagents, and common issues and solutions when using ion-pairing chromatography. -
Amino (NH2) column: How to keep high performance?
Introduce the principle of amino columns (nh2 columns) and the guidelines for optimizing amino column performance in reversed-phase and normal-phase mode. -
Does Smaller Column Particle Size Always Lead to Better Resolution in Liquid Chromatography?
In this article, we explore how particle size affects resolution in liquid chromatography through two real-world applications, and analyze why these cases show different results from common belief. -
What is the Dead Volume of A Column?
This article introduces dead volume in HPLC: its definition, calculation methods, and tips to reduce it for better peak shapes and chromatographic efficiency. -
Optimizing Phosphate Buffer Solution: Preparation Methods and Case Studies
This article covers the role of phosphate buffer solutions in maintaining pH stability for HPLC, detailing preparation methods that enhance consistency in peak separation results.
