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Gel Purification Chromatography
Gel Purification Chromatography Gel chromatography is a separation and analysis technology developed in the early 1960s. According to whether the separated objects are water-soluble substances, gel chromatography can be divided... -
How Can Polar Compounds be Better Retained?
Reversed-phase columns usually take silica gel, organic/ inorganic hybrid silica gel or polymer as the matrix, while C18 as the bonded phase. Based on the hydrophobic interaction between analytes and... -
Distinction of Related Concepts About Liquid Chromatography
High performance liquid chromatography (HPLC) is a fundamental analytical discipline with many similar concepts. Therefore, it’s necessary for us to learn how to distinguish some easily-confused concepts of liquid chromatography.... -
The principle of 100% water compatible column
There are many kinds of C18 columns in the market, some are 100% water compatible, some are not. Why can they be used at 100% water? How to distinguish? What... -
Chiral Separation of Remdesivir Isomers
2019-nCoV corona virus pandemic is exploding all over the world, how to cure it has become the most important and urgent matter at present. Remdesivir, developed by Gilead, which is... -
How to distinguish Guard column and Pre-column Inline Filter?
Guard column and Pre-column inline filter are commonly used in LC experiments. But a lot of users are confused about the two products as they look similar. How to distinguish?... -
Why is there always a "ghost" in your chromatogram?
What’s Ghost Peaks? In the chromatographic separation, there are some peaks which appears erratically like ghosts during gradient elution or long-period operation, we call them Ghost Peaks. The harm of... -
C18 under 100% water phase? No problem!
C18 is retentive to non-polar compounds as the non-polarity of C18 group, but for polar compounds, sometimes the mobile phase with high water ratio should be used to make the... -
Isomer Separation? Welch columns bring help
It’s usually very difficult to identify the twins, but mothers can easily identify their twins according to habits and characteristics. Isomer In Chemical world, there also exist some compounds with... -
Isomer Separation? Try Chiral columns
Isomers are compounds with similar structures and their chemical equilibrium constant(K) is almost the same. It’s very difficult to operate chromatography separation on isomers, especially the separation of meta and... -
Core-shell column：get high efficiency at low pressure
Superior core-shell column There are mainly two kinds of chromatographic packing materials: silica gel and polymer. Their structural difference makes maintenance and separation results different. Silica based packing materials contain... -
Batch to Batch Reproducibility? The solution is here!
Today we will bring “Solutions to solve poor reproducibility between batch to batch”. Q How to ensure good reproducibility when a new validated HPLC method is transferred to Q.C. lab?...